Is hsdRMS- also dam-?

Julia Molitor jmolitor at ns.cec.uchile.cl
Tue Nov 27 10:52:01 EST 2001


Hi netters,

I have problems in cleaving my vector with ClaI, I assume it is due to
methylation of the restriction site. Therefore I want to transform the
DNA from coli TOPO cells into a dam- strain, but I have only a hsdRMS-
genotype. Does this mean that the strain is also dam-?

Thanks,

Julia Molitor

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