Is hsdRMS- also dam-?

Duncan Clark junk at
Tue Nov 27 11:02:06 EST 2001

In message <3C03C41C.EC3952E9 at>, Julia Molitor 
<jmolitor at> writes on the Tue, 27 Nov 2001
>Therefore I want to transform the
>DNA from coli TOPO cells into a dam- strain, but I have only a hsdRMS-
>genotype. Does this mean that the strain is also dam-?


hsdRMS specifies EcoK restriction/methylation (for an E.coli K12 strain 
or EcoB for E.coli B strain etc.). Dam and dcm are separate methylation.

The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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