Is hsdRMS- also dam-?

Duncan Clark junk at 127.0.0.1
Tue Nov 27 11:02:06 EST 2001


In message <3C03C41C.EC3952E9 at tamarugo.cec.uchile.cl>, Julia Molitor 
<jmolitor at ns.cec.uchile.cl> writes on the Tue, 27 Nov 2001
>Therefore I want to transform the
>DNA from coli TOPO cells into a dam- strain, but I have only a hsdRMS-
>genotype. Does this mean that the strain is also dam-?

No.

hsdRMS specifies EcoK restriction/methylation (for an E.coli K12 strain 
or EcoB for E.coli B strain etc.). Dam and dcm are separate methylation.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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