Is hsdRMS- also dam-?

hroychow at NMSU.Edu hroychow at NMSU.Edu
Tue Nov 27 11:34:26 EST 2001


First of all, the dam/dcm systems are not what the hsdRMS  signifies.
Secondly, you should be able to find out about the genotype of the strain
you are using from the supplier.

Hiranya S Roychowdhury, Ph.D.
Coll. Asst. Prof.
Molecular Biology
Chemitry & Biochemistry
New Mexico State University
New Mexico, US,

On 27 Nov 2001, Julia Molitor wrote:

> Hi netters,
> 
> I have problems in cleaving my vector with ClaI, I assume it is due to
> methylation of the restriction site. Therefore I want to transform the
> DNA from coli TOPO cells into a dam- strain, but I have only a hsdRMS-
> genotype. Does this mean that the strain is also dam-?
> 
> Thanks,
> 
> Julia Molitor
> 
> ---
> 
> 


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