Is hsdRMS- also dam-?

Wolfgang Schechinger wolfsc at
Tue Nov 27 11:50:20 EST 2001


check somewhere in the back of NEB's catalogue. There is a list of suitable 



> Hi netters,
> I have problems in cleaving my vector with ClaI, I assume it is due to
> methylation of the restriction site. Therefore I want to transform the
> DNA from coli TOPO cells into a dam- strain, but I have only a hsdRMS-
> genotype. Does this mean that the strain is also dam-?
> Thanks,
> Julia Molitor
> ---

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a brain is reqired for decoding.
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw


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