Antibody affinity in Western blotting

Martin Smith martin_smith at sympatico.ca
Wed Nov 28 01:20:54 EST 2001


Hello,
I was wondering if antibody affinity for nonsequential epitopes on SDS
mercaptoethanool linearized and electrotransfered polypeptides is a
limitation of western blotting? Is it possible that unmarked polypeptides on
final membrane cannot be recognized by certain serum antibodies for such
peptides?
I cannot seem to find any reference to this in textbooks and newsgroups.
Thx in advance





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