local charges of peptide chain

[Wolfgang Schechinger]

Dear Martin, 

Thanks a lot for your suggestion.

Tryptic fragments still are too big: I really need the change in the charge of 
every single individual position (will mainly affect YDEHRK ;-) when pH 
changes.

Regards, 
Wo

> wouldnt digesting your protein with trypsin then isoelectric focusing do it?
> 
> ""Wolfgang Schechinger"" <wolfsc at ibms.sinica.edu.tw> wrote in message
> news:3C04F849.30179.8BBC518 at localhost...
> > Hi all,
> >
> > I would like to compare local charges of related proteins at different pH
> > (e.g. if Tyrosines are likely to be protonated or not at a certain pH).
> > A series of plots showing LOCAL positive/negative/zero charge is what I
> would
> > like to get. But I am NOT looking for a standard isoelectric plot since
> that
> > would only refer to the overall net charge.
> >
> > Before I start programming, is anyone aware of such a tool?
> >
> > Thanks and Regards,
> >
> > Wo
> >
> > -----
> > Dr. Wolfgang Schechinger
> > Institute of Biomedical Sciences
> > Academia Sinica, Taipei, Taiwan R.O.C.
> > e mail wolfsc at ibms dot sinica dot edu dot tw
> > -----
> >
> > ---
> 
> 
> 


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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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