Reference for the Minipreparation of plasmid DNA (Rusconi mix)

Markus Klein markus.klein at bota.unine.ch
Wed Nov 28 06:38:56 EST 2001


hi,

i am not sure but i thought this or a comparable method appeared in
technical tips online that you can find on the biomednet pages
(http://research.bmn.com/tto).

cu, markus

--
Markus Klein
Université de Neuchatel
Laboratoire de Physiologie végétale
Rue Emile Argand 13
CH-2007 Neuchatel - Switzerland
___________________________________
Phone: (+41) 32 - 718 - 2237
Fax: (+41) 32 - 718 - 2271
E-Mail: markus.klein at bota.unine.ch



Dani Hiestand wrote:

> hi
>
> I am desperately looking for the reference for the plasmid DNA
> minipreparation method which uses the so called Rusconi mix:
>
> 500ul Lysozyme (10mg/ml)
> 500ul 0.5M EDTA
> 250ul 1M Tris pH 7.5
> 100ul RNase (10mg/ml)
> 1.15ml Glycerol (87%)
> 0.02%Bromophenol blue (endconc. of 2mg/10ml)
> 7.5ml H2O
>
> store at -20 in aliquots
>
> suspend 1 colony in 12-15ul
> let stand at RT 10-15 min
> add 2ul Phenol, vortex, spin 2'
>
> Load aqueous supernatant on gel
>
> If anyone knows it, please provide me with the needed information.
>
> Thanks in advance
>
> Dani Hiestand
>
> Laboratory for Food Microbiology
> Institute for Food Science
> Swiss Federal Institute of Technology Zurich
>
> ---




More information about the Methods mailing list