cos cells tranfection using PEI

Cornelius Krasel krasel at
Tue Oct 2 01:06:31 EST 2001

Cornelius Krasel <krasel at> wrote:
> TINA <anfortin at> wrote:
>> About HEK-293 cells ... is it possible to transfect these cells by the
>> Fugene 6 method using a pSVL expression vector ?
> It's possible to transfect HEK293 cells with Fugene 6. We get very
> high transfection efficiencies using this method, but for routine
> transfection we stick to CaPO4.

Since I was asked in email what I consider a high transfection

with Fugene 6, we get at least 50% of the HEK293 cells transfected. It
sometimes also happens with CaPO4, but routinely the efficiency we get
there is more like 20-30%, and it is less reproducible than with Fugene
6 (especially when you have made up a new batch of buffer).


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at */
/* "Science is the game we play with God to find out what His rules are."  */

More information about the Methods mailing list