plant DNA quick screening

WuTomek wywal_to.gasior at friko.onet.pl
Tue Oct 2 03:55:12 EST 2001


On Mon, 01 Oct 2001 11:43:49 -0400, "Bryan J. Maloney"
<bjm10 at cornell.edu> wrote:

>In article <q433rtc2bg8hlmuv3nf5h84j7991gaai0j at 4ax.com>, WuTomek 
><wywal_to.gasior at friko.onet.pl> wrote:
>
>> I`m looking for fast method of DNA screening in potato plants. With
>> bacteria I have done screening with PCR of whole cells (first step is
>> 10 min heating in 94  C deg.). Is that possible with plants, or I need
>> to prepare DNA with phenol method ?
>
>As far as we can tell with our potato work, the DNA has to be extracted 
>and purified to at least some degree.

Thanks. Yesterday I tried do that with spinach plants. It doesn`t work
- some purification is necessary.
--
Pozdrowienia

  Tomek




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