No signal

Giselle gmartinez_noel at
Mon Oct 1 20:43:04 EST 2001

I have a fragment of 200pb (homolog cDNA) that I used as a probe for Northen
and Southern. I did the hybridization at 60 and 65 degrees and I washed with
high and low astringency but I had no signals. I only had the signal of the
positive control!. What can I do?. Thanks in advance.


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