No signal

Dr. Frank Sehlmeyer 0622755853-0003 at t-online.de
Tue Oct 2 10:09:56 EST 2001


Hi Giselle,

how did you label the cDNA ?
Did you started washing with low stringency ?
Which washing buffer you did use ?
May be I can help you then.

Greetings,

Frank

Giselle <gmartinez_noel at hotmail.com> schrieb in im Newsbeitrag:
20011002014304.20361.qmail at ww02.jatek.com...
> I have a fragment of 200pb (homolog cDNA) that I used as a probe for
Northen
> and Southern. I did the hybridization at 60 and 65 degrees and I washed
with
> high and low astringency but I had no signals. I only had the signal of
the
> positive control!. What can I do?. Thanks in advance.
>
>
> <http://www.biowww.net/forum/read.php?f=1&i=3999&t=3999>
>





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