Tth RT only with GSP possible?

Dr. Frank Sehlmeyer 0622755853-0003 at t-online.de
Tue Oct 2 10:26:33 EST 2001


Dear Nicolas,


of course you may take oligo-dT-primer for this.
If you take 5´-dT(18)-N-3´ or 5´-dT(18)-N-N-3´ or even 5´-dT(18)-N-N-N-3´
... primers you may level up the annealing temp.
N = random A, C, G or T

Greetings and have a great day,


Frank
emove the [nospam] (Nicolas von Ahsen) <nvahsen at hot[nospam]mail.com> schrieb
in im Newsbeitrag: 3bb8ac41.4477327 at news.gwdg.de...
> Dear all,
>
> I'm only aware of methods for RT with the Tth enzyme that use a gene
> specific primer. Must this be or is oligo-dT also possible? One could
> simply make the primer longer to accomodate with higher
> annealing/extension temperatures.
>
> Thank you.
> Nicolas
>





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