Primer dimer-really serious?

Linan Chen lichen at delphi.bsd.uchicago.edu
Tue Oct 2 18:16:54 EST 2001


hi,

Sounds very stupid. It is the common sense to avoid forming dimer when
design PCR primers. But is it really serious that two primers for pcr form
dimer? I doubt it because the high temp in the PCR cycle will sure destroy
the dimer. Am I right?

thanks,

linan
Chen, Linan

University of Chicago

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