293 cells and Zeocin selection

Baoshan bszhang at wsu.edu
Wed Oct 3 11:04:48 EST 2001

293 cells and Zeocin selectionI just got an reply from the Invitrogen, they said the optimum concentrations between 300-450 ug/mL. But this still should not prevent you from doing your own kill-curve, as you know different conditions and lab environments are able to sway the mechanism for the uptake of the antibiotic, therefore effecting the death of the cells. 

"Bao Shan Zhang" <zhang at vetmed.wsu.edu> wrote in message news:4FE6AC861731D511BCAD009027F64F58010E814C at cvm.vetmed.wsu.edu...

  Does anyone have an idea about the range of Zeocin concentration to select in HEK 293
  Thanks to all in advance, 
  Dept Vet Micro/Path 
  bszhang at wsu.edu 

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