293 cells and Zeocin selection

[Baoshan]

293 cells and Zeocin selectionI just got an reply from the Invitrogen, they said the optimum concentrations between 300-450 ug/mL. But this still should not prevent you from doing your own kill-curve, as you know different conditions and lab environments are able to sway the mechanism for the uptake of the antibiotic, therefore effecting the death of the cells. 



"Bao Shan Zhang" <zhang at vetmed.wsu.edu> wrote in message news:4FE6AC861731D511BCAD009027F64F58010E814C at cvm.vetmed.wsu.edu...

  Does anyone have an idea about the range of Zeocin concentration to select in HEK 293
  cells? 
  Thanks to all in advance, 
  baoshan 
  Dept Vet Micro/Path 
  WSU 
  bszhang at wsu.edu 



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