Immunohistochemistry - Leakage of protein from nucleus?
pd1 at mole.bio.cam.ac.uk
Thu Oct 4 12:31:38 EST 2001
Melissa Greeve wrote:
> Is this a logical explanation? Why would one protein act like this
> when fixed in 95% ethanol and not the other (both have similar
> cellular functions)? Can a PhD get any more complicated??
You could try formaldehyde fixation. 4% formaldehyde in PBS for 20 min
works well for a wide variety of antigens and cell types and in my hands
give sensible results for various nuclear antigens.
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