Immunohistochemistry - Leakage of protein from nucleus?

Paul pd1 at mole.bio.cam.ac.uk
Thu Oct 4 12:31:38 EST 2001



Melissa Greeve wrote:

> Is this a logical explanation? Why would one protein act like this
> when fixed in 95% ethanol and not the other (both have similar
> cellular functions)? Can a PhD get any more complicated??
>
>

You could try formaldehyde fixation.  4% formaldehyde in PBS for 20 min
at RT
works well for a wide variety of antigens and cell types and in my hands
give sensible results for various nuclear antigens.

Paul







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