transferring an IPG electrophoresis

Stefi~ xxxgifalone at tin.it
Fri Oct 5 12:26:46 EST 2001


"D.K." <dk at no.email.thankstospam.net> ha scritto nel messaggio
news:9pjedn$jda$1 at news.doit.wisc.edu...
> I'd suggest to put a layer of really thin filter paper between gel and
> membrane. Good quality paper will not bind proteins appreciably
> (not under electrotransfer conditions anyway) and if it is thin enough
> the drop of resolution will not be noticable.
>
> Alternatively, it _is_ possible to remove most of the low % PAAG stuck to
> the nitrocellulose under stream of water, but it takes a lot of patience.
>
> DK

Thank you for your suggestion. I'll try to put a very thin filter paper
between the gel and the nitro. If the things go well I'll advise you. Thx.
Stefano
xxxgifalone at tin.it
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