transferring an IPG electrophoresis

Stefi~ xxxgifalone at
Fri Oct 5 12:26:46 EST 2001

"D.K." <dk at> ha scritto nel messaggio
news:9pjedn$jda$1 at
> I'd suggest to put a layer of really thin filter paper between gel and
> membrane. Good quality paper will not bind proteins appreciably
> (not under electrotransfer conditions anyway) and if it is thin enough
> the drop of resolution will not be noticable.
> Alternatively, it _is_ possible to remove most of the low % PAAG stuck to
> the nitrocellulose under stream of water, but it takes a lot of patience.
> DK

Thank you for your suggestion. I'll try to put a very thin filter paper
between the gel and the nitro. If the things go well I'll advise you. Thx.
xxxgifalone at
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