GST-fusion elution problem

Deborah Otteson deotteson at jhmi.edu
Fri Oct 5 09:24:41 EST 2001


I have found a similar failure with elution of my GST-fusion proteins using
10mM glutathione/50mM Tris. In my case one of the problems was insufficient
buffering-resulting in a low pH. Increasing the tris to 100 mM greatly
facilitated elution.



<http://www.biowww.net/forum/read.php?f=1&i=4059&t=3944>




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