beta galactosidase assay
wolfsc at ibms.sinica.edu.tw
Mon Oct 8 01:26:56 EST 2001
could anyone give me some hints on the chemistry behind that assay for
beta galactosidase? It's for using beta gal as reporter gene assay.
the transfected cells are fixed with formaldehyde/glutardialdehude for 5 min
nad then washed with PBS (there is no further permeabilization)
and then incubated with a solution containing
X-Gal (of course)
potassium hexacyanoferrates (equimolar amounts of FeII and FeIII
hexacyanoferrate) in water.
this message consists of randomly assembled ascii characters
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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