beta galactosidase assay

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Mon Oct 8 01:26:56 EST 2001


Dear all, 

could anyone give me some hints on the chemistry behind that assay for 
beta galactosidase? It's for using beta gal as reporter gene assay.

the transfected cells are fixed with formaldehyde/glutardialdehude for 5 min 
nad then washed with PBS (there is no further permeabilization)

and then incubated with a solution containing 

X-Gal (of course)
magnesium chloride 
potassium hexacyanoferrates (equimolar amounts of FeII and FeIII 
hexacyanoferrate) in water.

any ideas?

Thanks, 
Wo
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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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