Binding curve analysis

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Tue Oct 9 05:21:17 EST 2001


Hi,

I have some binding isotherms (changes in fluorescence vs ligand 
concentration) and desperately need to work out the Kds . . .  Single 
binding site, Kd same order of magnitude as the protein conc. Using 
Kaleidagraph it should be possible to plug the data into a macro to do 
this, but I can't find one.  Anyone able to help?

Ta,

r

-- 
Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
               -- 'Let the beetles have their say' --




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