Binding curve analysis

[Richard P. Grant]

Hi,

I have some binding isotherms (changes in fluorescence vs ligand 
concentration) and desperately need to work out the Kds . . .  Single 
binding site, Kd same order of magnitude as the protein conc. Using 
Kaleidagraph it should be possible to plug the data into a macro to do 
this, but I can't find one.  Anyone able to help?

Ta,

r

-- 
Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
               -- 'Let the beetles have their say' --