Binding curve analysis

Richard P. Grant rpg14 at
Tue Oct 9 05:21:17 EST 2001


I have some binding isotherms (changes in fluorescence vs ligand 
concentration) and desperately need to work out the Kds . . .  Single 
binding site, Kd same order of magnitude as the protein conc. Using 
Kaleidagraph it should be possible to plug the data into a macro to do 
this, but I can't find one.  Anyone able to help?



Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at'
               -- 'Let the beetles have their say' --

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