Binding curve analysis
dk at no.email.thankstospam.net
Tue Oct 9 07:55:55 EST 2001
In article <rpg14-8080F6.11211609102001 at pegasus.csx.cam.ac.uk>, rpg at mrc-lmb.cam.ac.uk wrote:
>I have some binding isotherms (changes in fluorescence vs ligand
>concentration) and desperately need to work out the Kds . . . Single
>binding site, Kd same order of magnitude as the protein conc. Using
>Kaleidagraph it should be possible to plug the data into a macro to do
>this, but I can't find one. Anyone able to help?
Use any program that does least square non-linear regression.
I use Sigma Plot for DOS. As ridiculous as Excel is for anything
scientific, it also does it. A bunch of programs have trial versions
that work for some time - and you only need couple runs.
Microcal Origin and GraphPad Prism are such products (both
very good), Sigma Plot from Windows is OK, but I am not sure
if demo is fully funtional these days. There are certainly others.
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