Cloning into NgoMIV site and transformation troubl

Hakan hakan at icr.ac.uk
Tue Oct 9 12:20:12 EST 2001


I have been trying to insert cDNA with NgoMIV ends into a dammethylase
vector and cant get any colonies after transformation.
Has anyone else experienced this problem with either possible toxicity or
methylation problems in bacteria?
Tahnsk in advance for your time.
Hakan


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