Cloning into NgoMIV site and transformation troubl

Hakan hakan at
Tue Oct 9 12:20:12 EST 2001

I have been trying to insert cDNA with NgoMIV ends into a dammethylase
vector and cant get any colonies after transformation.
Has anyone else experienced this problem with either possible toxicity or
methylation problems in bacteria?
Tahnsk in advance for your time.


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