Brain Total RNA extraction

Bob Hartley r.hartley at
Fri Oct 12 04:45:11 EST 2001

In article <3bc6929f.745958 at>,
 wasowicz at (Krzysztof W. Wasowicz) wrote:

> On 11 Oct 2001 18:30:59 -0000, Adam <adam.kuhl at> wrote:
> >I am trying to extract total RNA from fish brain tissue using Trizol
> >reagent, but am getting very low A260/A28.  I have seen that this could be
> >due to high lipid content.  Can someone give me a good method of solving
> >this problem?
> >
> >Thanks,
> >Adam
> >
> >
> ><>
> >
> Hi,
> I have noticed that I am getting better results with an original
> method of Chomczynski & Sacchi (Anal Bioch - you can find reference
> from Medline probably - if not, write me). 

You might better using a GITC lysis then a seperate acid phenol 
chloroform extraction.

you might also consider using a tissue disruptor likre the bio101 
machine (also sold by Hybaid) OR rather than buying the machine you 
could buy the beads and use a whirlymixer.

also you could try freezing the lysis solution to -70/80 AND RAPIDLY 
THAWING IT IN A WATER bath (damn caps lock). :-)

you can make your own -70 desktop freezer for 10pounds ~16euros

the detais are available inthe archive. see posting by me Robert Hartley
rh at
Bob; Sunny Scotland

Robert Hartley
Spinal Cord Research Group
University of Glasgow. Glasgow G12 8QQ
mail: r.hartley at :

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