establish cell line

caulfield caulfield_z at yahoo.com
Tue Oct 16 04:36:41 EST 2001


Hi, does anyone have an easy protocol to help me establish cell lines
from peritoneal fluid, ascites of women with ovarian cancer.
I just try to put the fluid I get from the clinic directly in culture
but a whole array of unwanted RBC and WBC and fibroblasts grow while I
am only interested in cancer cells.
Any suggestions? Thanks




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