how can I set the parameter

[serotonin1]

<ryan> wrote in message news:20011017072036.16505.qmail at ww02.jatek.com...
> hi,all,
>
>        When I designed pcr primer by Oligo6, I got a result that the most
> stable 3'-dimer: 2bp,-1.3kcal/mol. Is that ok? And how much for 3bp, 4bp
> dimer? why they put a -  in front of kcal/mol?

To answer your question with a question, Is bond breakage a exothermic or
endothermic event?

Grady