separating myosin heavy chain isoforms

Nicole nicole_melbourne at
Thu Oct 18 20:48:02 EST 2001


I am looking for hints on how to improve the separation of myosin heavy
chain isoforms using SdS-PAGE.  Presently I am using a 35% glycerol and 9%
polyacrylamide gel (have tried concentration of acrylamide ranging from 7 to
9.4%).  I am running my samples in a fridge at 5C for 17-18 h.  My
separation is okay - but not very exciting.  Any suggestions.

Cheers, Nicole


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