khatipovNO at NOuchicago.edu
Thu Oct 18 21:32:14 EST 2001
There is a basic variety of EDTA brand named Trilon B. It dissolves in water
easily, but the resulting pH of the solution will be high. To dissolve
acidic EDTA, make a concentrated stock by slowly adding NaOH or NH4OH until
EDTA completely dissolves, adjust pH to ~8 and add to your buffer to a
desired concentration. At low concentration (1-10mM) EDTA will be soluble in
a relatively wide pH range (at least pH 6 and up).
"Neal" <nrmelvin at ucalgary.ca> wrote in message
news:3BCF7301.E6B59A42 at ucalgary.ca...
> I'm having trouble getting my EDTA to go into solution, even at 1mM in
> Tris-HCl, pH7.5... does heating help?? Is EDTA notoriously insoluble??
> Please email me directly as I rarely have access to newsgroups.
> Thanks in advance...
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