Aggregation of MDCK-II
fsafadi at lamar.colostate.edu
Sat Oct 20 12:36:28 EST 2001
Mauricio: I have been having the same problem with aggregation of the 3T3-L1
cells for Facs and was waiting to see what replies you get, However, I am so
close to figuring out a way to do it, I will give you the recipes when i make
sure it is working.
> "Alberto" <mrealpe at ibt.unam.mx> wrote in message
> news:Pine.LNX.4.10.10110111842130.25706-100000 at pbr322.ceingebi.unam.mx...
> Hello. For FACS purposes I have been culturing MDCK cells and deattaching
> them from the flask by means of EDTA during a relatively long period
> (around 50 minutes). After that when I proceed to count them they looks
> quite aggregated and this have been the cause to abort several further
> experiments, I have heard about certain substances useful to avoid this
> behaviour of the cell suspension, could anyone share with me their names
> and the easier way to get them ?. Thanxs in advance.
> Mauricio Realpe
Farida Safadi-Chamberlain, PH.D.
Department of Biochemistry
Colorado State University
Fort Collins, CO 80523
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