47004,247013 not in active file. Deleting. : I'm having trouble getting my EDTA to go into solution, even at 1mM in : Tris-HCl, pH7.5... does heating help?? Is EDTA notoriously insoluble?? : Please email me directly as I rarely have access to newsgroups. : Thanks in advance... : Neal Try to sonicate your solution or use sodium EDTA instead. Hope this help Ricky