FITC labelled primary antibody OK?

Martijn van Duijn m.v.duijn at amolf.nl
Mon Oct 22 08:58:53 EST 2001


Hi,

I have been using a FITC conjugated antibody against a HA tag in my 
favourite protein for microscopy purposes. However, I am now interested 
in trying a western blot using this antibody.
Has anyone tried using (FITC)-conjugated primary antibodies? Does the 
conjugate interfere with the recogition of the primary by the secondary 
antibody? I hope I can avoid buying the unlabelled version of the same 
antibody...

Thanks for any advice!

Martijn van Duijn




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