PCR problems?

Roland Hübner roland.hubner at ua.ac.be
Mon Oct 22 09:54:57 EST 2001

In article <3BD04A03.FE1C22D6 at ncsu.edu>, Liz Parks <liz_parks at ncsu.edu> 

> My lab mate, Jeanette Lyerly, and I wrote this after a depressing couple
> of weeks of PCR failures.  Hope you enjoy it!
> One day when I showed up for work
> My PCR had gone berserk.
> My samples wouldn't amplify,
> No matter how many things I tried.
> I finally had to ask it why,
> Why won't my samples amplify?
> Then came the following reply —
> I do not care how hard you try….
> I will not amp it on a plane,
> I will not amp it on a train.
> I will not amp it in the lab,
> I will not amp it in a cab.
> I will not amp it with new water,
> I will not amp it, so don't bother.
> I will not amp it in a plate,
> Even if your results are late.
> I will not amp with new buffer,
> I will not amp it before supper.
> You cannot run me in this lane,
> I am making you insane.
> I will not amp it with new dNTPs,
> I will not amp it if you sneeze.
> I will not amp it with this Taq,
> I do not like it, take it back.
> I will not amp it in this well,
> They'll put you in a padded cell.
> I do not like this new machine,
> I’ll only amp it in your dreams.
> I will not amplify your DNA,
> No matter what you do or say.
> And so it went, that's how it goes,
> The magic left us, I suppose,
> (Where it went, no one here knows).
> But if you find out where it went,
> Don't tell me now ‘cause I'm spent.
> I was here, but now I'm gone,
> I threw that machine out on the lawn.
> I decided to go and have some fun.
> Selling seashells in the sun.
> Now I have finished my silly poem,
> I give up, I'm going home.
>             -Jeanette Lyerly and Liz Parks
> --
> ~~~~~~~~~~~~~~~~~~~~~
> Liz Parks
> North Carolina State University
> Department of Plant Pathology
> Raleigh, NC  27695
> ~~~~~~~~~~~~~~~~~~~~~


 when you come back from home, just give "Rockstart" buffer a go --> 
www.klentaq.com/rockstart ... REALLY good stuff!


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