FITC labelled primary antibody OK?

[Henk Veldman]

Martijn van Duijn schreef:

> Hi,
>
> I have been using a FITC conjugated antibody against a HA tag in my
> favourite protein for microscopy purposes. However, I am now interested
> in trying a western blot using this antibody.
> Has anyone tried using (FITC)-conjugated primary antibodies? Does the
> conjugate interfere with the recogition of the primary by the secondary
> antibody? I hope I can avoid buying the unlabelled version of the same
> antibody...
>

You could try a HRP-labelled anti-FITC antibody as a second step. Since
FITC-labelled antibodies are so widely available, many of the antibody
companies produce them. I know Roche does sell one, just browse through
some catalogues.

Henk

--
H. Veldman
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)