FITC labelled primary antibody OK?

Henk Veldman H.Veldman-2 at
Tue Oct 23 01:45:16 EST 2001

Martijn van Duijn schreef:

> Hi,
> I have been using a FITC conjugated antibody against a HA tag in my
> favourite protein for microscopy purposes. However, I am now interested
> in trying a western blot using this antibody.
> Has anyone tried using (FITC)-conjugated primary antibodies? Does the
> conjugate interfere with the recogition of the primary by the secondary
> antibody? I hope I can avoid buying the unlabelled version of the same
> antibody...

You could try a HRP-labelled anti-FITC antibody as a second step. Since
FITC-labelled antibodies are so widely available, many of the antibody
companies produce them. I know Roche does sell one, just browse through
some catalogues.


H. Veldman
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)

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