FITC labelled primary antibody OK?

Peter Ashby p.r.ashby at
Tue Oct 23 05:11:02 EST 2001

In article <3BD511FC.95CDFDB5 at>,
 Henk Veldman <H.Veldman-2 at> wrote:

> Martijn van Duijn schreef:
> > Hi,
> >
> > I have been using a FITC conjugated antibody against a HA tag in my
> > favourite protein for microscopy purposes. However, I am now interested
> > in trying a western blot using this antibody.
> > Has anyone tried using (FITC)-conjugated primary antibodies? Does the
> > conjugate interfere with the recogition of the primary by the secondary
> > antibody? I hope I can avoid buying the unlabelled version of the same
> > antibody...
> >
> You could try a HRP-labelled anti-FITC antibody as a second step. Since
> FITC-labelled antibodies are so widely available, many of the antibody
> companies produce them. I know Roche does sell one, just browse through
> some catalogues.

their AP labelled anti-FITC antibody seems to be much more sensitive 
than the HRP one. In my hands at least.


Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.

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