phosphorothioate oligos as primers in PCR ?
wolfsc at ibms.sinica.edu.tw
Tue Oct 23 12:17:54 EST 2001
I once tried several (5 or 6) 17 to 19 mers, full PTOs. None ove them worked.
But I didn't try to do optimize, just was a little courious.
I'd expect the P=S bonds to cause some problems (killing the polymerase by
forming some sort of tight bonds as also known from ATPgammaS and kinases
But why not give it a try and tell us here?
> Hi there,
> does anyone know, whether phosphorothioate oligos will work as primers
> in PCR?
> Josmar Langner
> NOXXON Pharma AG
> Gustav-Meyer-Allee 25
> D-13355 Berlin
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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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