phosphorothioate oligos as primers in PCR ?

Roland Hubner roland.hubner at ua.ac.be
Tue Oct 23 13:50:57 EST 2001


> Hi there,
> 
> does anyone know, whether phosphorothioate oligos will work as primers
> in PCR?
> 
> Thanks

Josmar,

 in NAR, Skerra et al. published about the use of primers protected at 
3'-end by phosphorothioate links against the nibbling activity of 
proofreader DNA pols (Pfu)...

Hope this helps,
 Roland




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