phosphorothioate oligos as primers in PCR ?

Roland Hubner roland.hubner at
Tue Oct 23 13:50:57 EST 2001

> Hi there,
> does anyone know, whether phosphorothioate oligos will work as primers
> in PCR?
> Thanks


 in NAR, Skerra et al. published about the use of primers protected at 
3'-end by phosphorothioate links against the nibbling activity of 
proofreader DNA pols (Pfu)...

Hope this helps,

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