alkaline agarose gel...what concentration?

Stefi~ xxxgifalone at tin.it
Tue Oct 23 14:24:15 EST 2001


Hi everyone.
I'm trying to do an alkaline agarose gel (1,5%) but I obtain a very weak gel
(too easy to be broken). May I increase agarose concentration (4%)? Please
note that my next step is to do a capillary blotting with positively charged
nylon (I have to transfer fragments from 30 bp to 254 bp). Thanx so much!
Stefano
xxxgifalone at tin.it
(remove antispam xxx)




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