alkaline agarose gel...what concentration?
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Tue Oct 23 15:17:44 EST 2001
Are you melting the agarose in the presence of base? This definately makes
a soft gel.
The agarose should be melted in water, cooled to 65, then the base added
before pouring the gel. When I do it this way, 1% agarose makes a fairly
>I'm trying to do an alkaline agarose gel (1,5%) but I obtain a very weak gel
>(too easy to be broken). May I increase agarose concentration (4%)? Please
>note that my next step is to do a capillary blotting with positively charged
>nylon (I have to transfer fragments from 30 bp to 254 bp). Thanx so much!
>xxxgifalone at tin.it
>(remove antispam xxx)
Michael L. Sullivan, Ph.D
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax
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