alkaline agarose gel...what concentration?

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Tue Oct 23 15:17:44 EST 2001


Are you melting the agarose in the presence of base?  This definately makes
a soft gel.

The agarose should be melted in water, cooled to 65, then the base added
before pouring the gel.  When I do it this way, 1% agarose makes a fairly
firm gel.

Mike

>Hi everyone.
>I'm trying to do an alkaline agarose gel (1,5%) but I obtain a very weak gel
>(too easy to be broken). May I increase agarose concentration (4%)? Please
>note that my next step is to do a capillary blotting with positively charged
>nylon (I have to transfer fragments from 30 bp to 254 bp). Thanx so much!
>Stefano
>xxxgifalone at tin.it
>(remove antispam xxx)


Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax

---




More information about the Methods mailing list