phosphorothioate oligos as primers in PCR ?

Tim Fitzwater TFITZWATER at somalogic.COM
Tue Oct 23 17:33:34 EST 2001


The promega website reports "Another option to prevent primer degradation
(when using Pfu DNA polymerase which has 3' > 5' exo activity) is the
introduction of a single phosphorothioate-coupled base during synthesis of
the oligonucleotide (Skerra, A (1992) . Nucl. Acids res. 20, 3551)."


<http://www.biowww.net/forum/read.php?f=1&i=4274&t=4264>




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