SDS-PAGE Problem

dbell dbell at qnis.net
Tue Oct 23 18:13:11 EST 2001


Denis,
You might try buying new reagents
Acrylamide can go bad, as can TEMED and ammonium persulfate

Deanne Bell

USDA Agricultural Research Service
9611 S. Riverbend Avenue
Parlier, CA 93648

(559) 596-2806
(559) 596-2791 fax
dbell at fresno.ars.usda.gov


On Tuesday, October 23, 2001 11:58 AM, user [SMTP:q6252397 at fernuni-hagen.de] wrote:
: Dear all,
: 
: I usually use a 13% acrylamide gel for protein electrophoresis. my samples
: are whole cell extract and a standard.
: Although I changed anything, the samples (and standard) on my last gels were
: running, as they would on a lower percentage gel.
: Has anybody an idea what might be the cause of this?
: 
: Thanks,
: Denis
: 
: 

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