about CG-rich amplification

[Duncan Clark]

In message <20011023040542.6391.qmail at ww02.jatek.com>, Tom 
<?@?.?.invalid> writes on the Tue, 23 Oct 2001
>      If CG is over 60% of my PCR product, how much DMSO should I add in my
>PCR buffer?  5%?

5 -10%. Try betaine to 1M final or 0.4M final sulfolane or 0.4M 
2-pyrrolidone or combination of  2-pyrrolidone and betaine.

Try a PCR mix with Taq and a proof-reading enzyme component with above.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382