about CG-rich amplification
d_clark at genesys.demon.co.uk
Wed Oct 24 05:07:14 EST 2001
In message <20011023040542.6391.qmail at ww02.jatek.com>, Tom
<?@?.?.invalid> writes on the Tue, 23 Oct 2001
> If CG is over 60% of my PCR product, how much DMSO should I add in my
>PCR buffer? 5%?
5 -10%. Try betaine to 1M final or 0.4M final sulfolane or 0.4M
2-pyrrolidone or combination of 2-pyrrolidone and betaine.
Try a PCR mix with Taq and a proof-reading enzyme component with above.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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