about CG-rich amplification

Duncan Clark d_clark at genesys.demon.co.uk
Wed Oct 24 05:07:14 EST 2001

In message <20011023040542.6391.qmail at ww02.jatek.com>, Tom 
<?@?.?.invalid> writes on the Tue, 23 Oct 2001
>      If CG is over 60% of my PCR product, how much DMSO should I add in my
>PCR buffer?  5%?

5 -10%. Try betaine to 1M final or 0.4M final sulfolane or 0.4M 
2-pyrrolidone or combination of  2-pyrrolidone and betaine.

Try a PCR mix with Taq and a proof-reading enzyme component with above.

The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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