SDS-PAGE Problem

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Oct 24 06:21:06 EST 2001


Denis,
     are you making your own gels?  Has the acrylamide or bis gone off?
Once my bis went off (the powder in the reagent jar!) and it made my gels
perform very badly: as if they were low concentration gels.  Mike.

 On Tue, 23 Oct 2001, user wrote:

> Dear all,
>
> I usually use a 13% acrylamide gel for protein electrophoresis. my samples
> are whole cell extract and a standard.
> Although I changed anything, the samples (and standard) on my last gels were
> running, as they would on a lower percentage gel.
> Has anybody an idea what might be the cause of this?
>
> Thanks,
> Denis
>
>
>




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