SDS-PAGE Problem

Debora Fontanini dfontani at facstaff.wisc.edu
Wed Oct 24 09:48:09 EST 2001


Hello Denis,

I never had problems with TEMED although we have been using years old 
solutions.  Mostly we have been having your kind of results when someone 
has made a mistake in preparing the running buffer.  An incorrect Tris 
concentration or pH can lead to the proteins migrating faster, just like on 
a lower percentage gel.

Debora

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