strange precipitate on western blot

Wolfgang Schechinger wolfsc at
Thu Oct 25 04:40:50 EST 2001

Dear Ricky, 

Are you using powdered gloves?


> Dear friends,
> we have raised this antiserum against our protein by immunizing a little
> bunny. However, when we do western blot with this serum all we see are 
> millions of little spots on the membrane but no specific signal. Looks
> like
> something precipitated onto the membrane resembling kind of hot spots
> in northern blots. We checked all our reagents but it seems that this
> phenomenon only occurs with this specific antiserum. Have you ever
> observed
> something like this and how to solve this problem?
> Any input welcome.
> Best wishes
> Ricky

disclaimer: this message consists of 
randomly assembled ascii characters.
a brain is reqired for decoding.
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw


More information about the Methods mailing list