strange precipitate on western blot

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Thu Oct 25 04:40:50 EST 2001


Dear Ricky, 

Are you using powdered gloves?

Wo


> Dear friends,
> 
> we have raised this antiserum against our protein by immunizing a little
> bunny. However, when we do western blot with this serum all we see are 
> millions of little spots on the membrane but no specific signal. Looks
> like
> something precipitated onto the membrane resembling kind of hot spots
> in northern blots. We checked all our reagents but it seems that this
> phenomenon only occurs with this specific antiserum. Have you ever
> observed
> something like this and how to solve this problem?
> Any input welcome.
> Best wishes
> Ricky
> 
> 


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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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