phosphorothioate oligos as primers in PCR

Phil Sanders Phil.Sanders at
Fri Oct 26 11:08:43 EST 2001

I tried a PCR with 1 oligo containing all phosphorothioates and 1 normal
oligo and that didn't work despite various optimising attempts. The only
way I got it to work was to have the part of the oligo that was
complementary to the template containing normal nucleotides and then to
have a 5' noncomplementary region and it was in this that I put the
phosphorothioates and that worked just fine.
Hope that helps,


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