Ian A. York
iayork at panix.com
Mon Oct 29 06:50:22 EST 2001
In article <B8022F8F.223%takedatu at med.kurume-u.ac.jp>,
takedatu <takedatu at med.kurume-u.ac.jp> wrote:
>Have anybody tried to test the efficiency of transfection especially Cos7
>cells ? polyfectamine or lipofectamine plus or lipofectamine gives a similar
>reslt? or which one would be better to use with Cos7 cells. and could
Fugene 6 gives me much better efficiency (80% or more) than polyfectamine
or lipofectamine plus (both around 50%). Lipofectamine 2000 gave
efficiency similar to Fugene 6, but also had much higher toxicity.
Just follow the manufacturer's directions.
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
More information about the Methods