pre-running discont SDS gels???

Nick Theodorakis nicholas_theodorakis at
Mon Oct 29 22:30:27 EST 2001

In <3BDE8522.10549.247BF76 at localhost>, Wolfgang Schechinger wrote:
>Hi all, 
>I noticed that some people pre-run discontinuous SDS gels (where stacking and 
>separating gel have different pH in their respective buffers). According to 
>Maniatis one should not do that because it will kill the pH difference and in 
>my experience it is not necessary.
>I don't want to make this experiment, so I better ask here.
>Any opinions? 

I've never done it and I have never heard of anybody doing it, and the
explanation you gave makes sense to me.


Nick Theodorakis
nicholas_theodorakis at


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