intact mitochondria

Matthias Schauen m.schauen at
Tue Oct 30 06:46:26 EST 2001

Hi jayakumar,

a good article with isolation protocols is

Rickwood D, Wilson MT and Darley-Usmar VM (1987). Isolation and characteristics
of intact mitochondria, in: Mitochondria: a practical approach (Darley-Usmar VM,
Rickwood D und Wilson MT, eds.), 1-16, IRL Press Oxford, England

The article also describes the measurement of mitochondrial respiration with an
oxygen electrode. The  respiratory control ratio RCR is a good indicator of
intactness of the mitochondrial inner membrane.

Several other isolation protocols can be found in Methods in Enzymology Vol LV.

Good luck,


Matthias Schauen    Fon: ++49-0221-4706476
Institut fuer Biochemie   Fax: ++49-0221-4705091
Zuelpicher Str. 47, 3. OG   e-mail: m.schauen at
D-50674 Koeln

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