T4-pol PstI/Exo EcoRI/fill in

Tom Knight tk at suspiria.ai.mit.edu
Tue Oct 30 09:52:21 EST 2001


Have you considered making a pair of primers containing the exact
restriction site you'd like to see, and doing PCR around the vector,
followed by a cut and religation?  I've found that to be a more
reliable approach, in general.

Ricky Boernke <ricky_boernke at gmx.net> writes:
> I would like to remove all restriction sites but the HindIII site from
> the pUC18 polylinker region.




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