PCR with smear

dbell dbell at qnis.net
Tue Oct 30 10:51:29 EST 2001

On Monday, October 29, 2001 7:16 PM, Peter Guo 
[SMTP:yuyuanguo at students.wisc.edu] wrote:
: Dear friends:
: Does somebody know why the PER from plant DNA ( from transgenic plants )
: got smear? I got positive control and it gives a band.
: Peter
Hi Peter

Without knowing more about what you are doing, I would say it is either due 
to poor primer annealing or [Mg] is out of whack.  I would try increasing 
the annealing temperature of your PCR by increments of 2 - 3 degrees.  If 
that didn't work, try doing a [Mg] gradient.

Deanne Bell

USDA Agricultural Research Service
9611 S. Riverbend Avenue
Parlier, CA 93648

(559) 596-2806
(559) 596-2791 fax
dbell at fresno.ars.usda.gov


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