Protease-deficient amber supressor

James james_hallinan at hotmail.com
Wed Oct 31 05:53:30 EST 2001


Hi all,

We're doing phage display in TG1s and are having some proteolysis
problems.  Does anyone know of a protease deficient (ompT, lon) amber
supressor (supE, glnV) strain?  One that is recA- would be even
better.  I've tried NEB and Maniatis with not too much sucess.

Thanks




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