about CG-rich amplification
paszkiet at xecu.net
Wed Oct 31 13:30:40 EST 2001
(Tom) wrote in <20011023040542.6391.qmail at ww02.jatek.com>:
> If CG is over 60% of my PCR product, how much DMSO should I add in
>PCR buffer? 5%?
You could also try 5-10% glycerol. I had success using 10% glycerol,
amplifying regions from viral DNA that is 75% GC.
More information about the Methods