about CG-rich amplification

Brian Paszkiet paszkiet at xecu.net
Wed Oct 31 13:30:40 EST 2001


 (Tom) wrote in <20011023040542.6391.qmail at ww02.jatek.com>:

>hi,all,
>
>
>
>      If CG is over 60% of my PCR product, how much DMSO should I add in
>      my 
>PCR buffer?  5%?
>
>
>      thanks
>
>
><http://www.biowww.net/forum/read.php?f=1&i=4256&t=4256>

You could also try 5-10% glycerol.  I had success using 10% glycerol, 
amplifying regions from viral DNA that is 75% GC.

Brian P.




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